摘要
于2013年4月从宁德患内脏白点病大黄鱼(Pseudosciaena crocea)中分离得到两株优势菌NZBD9和NZBD11,这两株菌在16—19°C条件下回归感染能引起大黄鱼内脏白点病,而在7—10°C和24—27°C条件下同样的人工感染不能致病,从而证实这两株菌为大黄鱼内脏白点病的病原菌。经16S rDNA基因的测序和时间飞行质谱微生物鉴定仪分析,NZBD9和NZBD11同为变形假单胞菌。药敏性实验结果显示NZBD9对庆大霉素、诺氟沙星和四环素等7种药物高度敏感。组织病理学观察结果显示病鱼的肝脏、脾脏、头肾等组织中均出现明显病症,如变性和坏死。
Two dominant strains(NZBD9 and NZBD11) were isolated from internal organs of Pseudosciaena crocea suffered from white-spots disease in April 2013. Both strains could cause the disease artificially in the internal organs in 16—19℃ but at 7—10℃ and 24—27℃. The results indicate that the two strains were the causative agent of the disease in farmed P. crocea. Both were identified as Pseudomonas plecoglossicida by time-of-flight mass spectrometer in 16S rDNA sequences. Experiments in susceptibility showed that NZBD9 is highly sensitive to 7 drugs including gentamicin, norfloxacin, and tetracycline. Histopathological observation showed that affected fish had clear symptoms of degeneration and necrosis in liver, kidney and spleen.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2014年第2期409-417,共9页
Oceanologia Et Limnologia Sinica
基金
公益性行业(农业)科研专项
200903029号
国家自然科学基金项目
31272699号
41176115号
关键词
大黄鱼
变形假单胞菌
内脏白点病
致病性
Pseudosciaena crocea
Pseudomonas plecoglossicida
pathogenicity
internal organs white-spots disease
