摘要
比较了马立克病毒超强毒株rMd5对中国3种不同地方品系鸡(济宁百日鸡、汶上芦花鸡、寿光黑鸡)的致病性。将rMd5分别腹腔接种1日龄(500 PFU·只^-1)和7日龄(1500 PFU·只^-1)不同品系鸡并饲养90 d。结果表明rMd5均能够引起3种地方品系鸡的特异性马立克病变,但是致病性的强度差异显著:寿光黑鸡的死亡率和肿瘤发生率明显高于其他2种鸡,几乎和SPF鸡类似,而且rMd5在寿光黑鸡群中的横向传播能力最强;济宁百日鸡和汶上芦花鸡相比对rMd5的抗感染和抗病能力强。因此,济宁百日鸡和汶上芦花鸡可作为遗传抗性基因的筛选和遗传抗性机制研究的候选品系。
This experiment was conducted to study the pathogenicity of very virulent MDV strain rMd5 between three different local chicken lines(Ji-ning-bai-ri,Wen-shang-lu-hua,Shou-guanghei) in China.rMd5 strain was inoculated to chickens by intraperitoneal injection,1-day-old chicken with 500 PFU and 7-day-old chicken with 1 500 PFU.The feeding trial lasted for 90days.rMd5 causes specific lesions of Marek's disease in three different local chicken lines,but the degree of the pathogenicity differs significantly:the mortality and oncogenicity of Shouguang-hei are significantly higher than the other two chicken lines,which is almost similar to SPF chicken.In addition,the transmission capacity of rMd5 in Shou-guang-hei is the highest;By contrast,the resistance of Ji-ning-bai-ri and Wen-shang-lu-hua lines to rMd5 infection and disease are greater.Therefore,Ji-ning-bai-ri and Wen-shang-lu-hua can be candidate lines to screen for genetic resistance genes and study genetic resistance mechanism.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第1期101-106,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31072149)
关键词
马立克病
地方品系鸡
遗传抗性
Marek's disease
local chicken lines
genetic resistance
