鸡嗉囊中部分免疫细胞的分布定位研究

Study on Location and Distribution of Several Immunocytes in Crop of Chicken

旨在应用组织学和免疫组织化学技术,观察鸡嗦囊中的免疫细胞——T淋巴细胞及其亚群、B淋巴细胞、单核细胞和抗原呈递细胞的定位分布与数量情况,在组织和细胞水平上分析鸡嗉囊的免疫功能和建立情况。结果显示:嗉囊的黏膜固有层中分布大量的淋巴组织,Bu-1b^+细胞主要分布在生发中心中,CD3^+、CD4^+、CD8^+T淋巴细胞较多地分布于弥散的淋巴组织及生发中心周围的滤泡间区域。部分CD3^+、CD8^+细胞和MHC-Ⅱ^+细胞甚至浸润到黏膜上皮与腺上皮中,使其转变为淋巴上皮。Ma^+细胞少量的分布于固有层、黏膜下层及肌层;MHG-Ⅱ^+细胞则分布于整个组织中。研究表明:嗉囊作为鸡饱腹后储存食物的场所,其以固有层黏液腺分泌黏液而起到润湿及软化食物的作用;同时,它具有参与黏膜免疫的组织和细胞基础,是肠道相关淋巴组织(GALT)的重要组成部分。

Histological and Immunohistochemical techniques were used to observe the distribution and quantity of immunocytes,T lymphocytes and its subpopulation,B lymphocytes,Mononuclear/macrophage cells and APCs,then to analyze the immune function and establishment of the crop on histological and cellular level in chicken.The results showed;The lymphoid-tissues are located in the lamina propria at the junction of the crop and the cervical and thoracic parts of the esophagus.A significant count of CD3^+,CD4^+,CD8^+ T lymphocytes are evident in the diffused lymphoid tissues and the interfollicular areas around germinal center where Bu-1b^+cells are located in.Parts of CD3^+,CD8^+ cells and MHC-Ⅱ^+ cells are infiltrated to the epitheliums of the mucus and the mucosal glands,which are transformed to the lymphoepithelium;Ma^+ cells are slightly distributed in the Lamina propria submucosa muscular layer of mucosa and MHC- Ⅱ^+ emerged in all of the tissues.The results showed that as the first storage place of food in chicken,the crop could be responsible for Moisturizing and softening food through mucus from mucus-producing oesophageal glands in lamina propria;as well as posses histological and cellular capacity of attendant mucosal immunity.The crop is an important part of gut-associated lymphoid tissue(GALT).