白头翁汤对大鼠跨内皮迁移的中性粒细胞溶菌酶的影响

Effects of Pulsatilla Decoction on the Lysozyme of Transendothelial Migration of Polymorph Nuclear Neutrophils in Rat

为研究白头翁汤(PD)的抗菌机制,利用Transwell体系建立细菌脂多糖(LPS)诱导的白细胞迁移模型,在20μg·mL^-1PD作用下,采用琼脂糖火箭电泳和荧光测定的方法,对穿过和不穿过大鼠肠黏膜微血管内皮细胞(RIMECs)的中性粒细胞(PMNs)溶菌酶浓度和活性测定,并对测定结果进行统计学检验。结果表明跨内皮迁移的PMNs中,PD+L,PS组与对照组、LPS组和PD组相比溶菌酶浓度极显著升高(P<0.01),其溶菌酶活性显著升高(P<0.05);不跨内皮迁移的PMNs各组溶菌酶浓度和活性无显著差异(P>0.05)。结果表明白头翁汤治疗动物细菌性疾病机理之一是与其保护RIMECs不受LPS损伤和激活跨内皮PMNs溶菌酶密切相关。

To investigate the antibacteria mechanism of Pulsatilla decoction,the Transwell system was used to establish the model of leukocyte migration induced by Lipopolysaccharide(LPS).The lysozyme concentrations and activity of the polymorph nuclear neutrophils(PMNs),which could pass through the rat intestinal mucosa microvascular endothelial cells(RIMECs) and not,were tested by the means of Agarose rocket electrophoresis and Fluorescence.The results showed that among the transendothelial migration of PMNs,the lysozyme concentrations of the 'PD +LPS' group(compared with the control group) and the LPS group(compared with the PD group) significantly increased(P<0.01),and their lysozyme activity also increased significantly(P<0.05).There is no significant difference in the groups among the no transendothelial PMNs(P>0.05).The results indicated that one of the antibacterial mechanism of PD is closely related with the protection against LPS and activation of transendotelial migration of PMNs lysozyme.