GnRH受体在雌性山羊肠系膜后神经节中的分布

Immunohistochemical Localization of GnRHR on Caudal Mesenteric Ganglion in Female Goats

本试验旨在研究促性腺激素释放激素受体(GnRHR)在雌性山羊肠系膜后神经节(CMG)中的分布,以探讨CMG中存在的GnRHR在协调动物生殖活动中GnRH的内分泌调节与自主神经调节两种途径中的功能地位。试验采用免疫组织化学SP法,观察GnRHR在CMG中的分布特点。结果显示,在CMG中,神经元均为GnRHR免疫反应阳性细胞,其神经元细胞膜和细胞质呈棕黄色,GnRHR为强阳性。神经元突起呈黄色,GnRHR为中等阳性。卫星细胞和神经纤维中只有GnRHR弱阳性物质。图像分析表明,与其他非神经元成分相比,神经元胞体上GnRHR的相对表达量差异极显著(P<0.01)。结果表明:雌性山羊CMG中神经元胞体是GnRH发挥作用的主要靶细胞,提示GnRH有可能通过CMG的交感节后神经元上的GnRHR来调节该神经节所支配的生殖器官的功能活动,从而使CMG中存在的GnRHR成为协调生殖活动中GnRH内分泌调节与自主神经调节两种调节机制的功能中转站。

This experiment was conducted to detect existence of GnRHR in caudal mesenteric ganglion(CMG) of female goat,and discuss the possible functional role of GnRHR in regulating reproductive activity through endocrine and autonomic nerve pattern.Immunohistochemical SP method was used to observe the distribution characteristics of GnRHR in CMG.The results showed that all the neurons in CMG were responded positively to GnRHR.The membrane and whole cytoplasm of neurons were strong positive stained with brown and the neurite was middle positive stained with yellow to GnRHR.Weak GnRHR immunoreactivity products distributed on passing nerve fibers and satellite cells.Image analysis documented a significant difference of GnRHR expression between neurons and non-neurons(P<0.01).The results suggest that CMG's neurons are the major targets of GnRH,which implies GnRH may regulate the function of reproductive organs through the GnRHR distributed in CMG.Therefore GnRHR may be a 'transfer station' to coordinate reproductive activity through endocrine and autonomic nerve pattern.