转录因子Sox-5负调控猪S100A12基因表达

The Transcription of S100A12 Gene Negatively Regulated by Sox-5 in Pig

本研究旨在确定猪S100A12启动子-1 013^-590区域中起负调控作用的转录因子。首先,通过双荧光素酶报告系统对猪S100A12启动子-1 013^-590区域转录活性进行研究,分别对S100A12启动子-1 013^+598、-931^+598、-830^+598、-711^+598和-590^+598转录活性进行检测。然后对-1 013^-931关键区域进行进一步缺失及突变研究,鉴定该区域负调控S100A12基因的转录因子及其结合位点。结果表明,猪S100A12启动子-1 013^-931区域缺失后,转录活性显著上升,表明该区域存在负调控猪S100A12基因转录因子结合位点。生物信息学手段预测发现,在-1 013^-931区域内存在1个Sox-5,2个GATA-1转录因子结合位点。当转录因子Sox-5结合位点进行缺失及突变时,猪S100A12转录活性显著上升。结果显示转录因子Sox-5负调控猪S100A12基因的表达。

This study aimed to investigate the negative transcriptional factors and their binding motifs in the promoter region of porcine S100A12gene.The promoter transcriptional activity of the regions,which contains-1 013-+598,-931-+598,-830-+598,-711-+598and-590-+598were detected by dual luciferase assay system.Furthermore,the transcriptional activity and the transcriptional factors' binding sites located in the-1 013--931region was deeply investigated through DNA deletion and mutation analyses.Porcine S100A12promoter activity decreased significantly(P<0.05)when the region-1 013--931was deleted.Bioinformatics analyses results showed that transcriptional factor Sox-5and GATA-1binding sites located in this region. The promoter activity was significantly increased only when the binding site of Sox-5was mutant or deleted.The results indicate that the expression of S100A12gene is negatively regulated by the transcriptional factor Sox-5.