抑制整合素α9基因的表达对黑色素瘤细胞B16F1的生长和肺转移的影响

Effects of shRNA-mediated Silencing of Integrin α9 Expression on Growth and Lung Metastasis of B16F1 Melanoma Cells

目的观察shRNA干扰整合素α9(ITGA9)的表达对黑色素瘤细胞B16F1的生长和肺转移的影响。方法用RNA干扰技术下调B16F1中ITGA9的表达,建立小鼠皮下成瘤和肺转移模型,观察肿瘤生长情况,计数肺转移灶数量。结果 ITGA9-shRNA转染组的肿瘤生长速度减慢(P<0.05),实验终点,该组肿瘤平均体积与scramble-shRNA组相比下降36%;肺转移灶数量显著减少(P<0.05)。结论下调ITGA9的表达可抑制黑色素瘤细胞B16F1在小鼠体内的生长和肺转移。ITGA9可能成为黑色素瘤的治疗靶点。

Objective To investigate the effects of shRNA-mediated silencing of integrin α9(ITGA9) expression on growth and lung metastasis of B16F1 melanoma cells. Methods ITGA9-shRNA plasmid was used to inhibit the expression of ITGA9 in melanoma cell line B16F1. The effectiveness and feasibility of RNA interference were confirmed by RT-PCR and Western blot. Subcutaneous tumor model and lung metastasis model were successfully established on C57BL/6 mouse for observation of tumor growth and the number of lung metastatic foci. Results Tumor growth was slowed down in ITGA9-shRNA(P<0.05).The ratio of average tumor volume between shRNA group and scramble-shRNA group was decreased by 36%. The number of lung metastatic foci was significantly decreased(P<0.05). Conclusion Down-regulation of ITGA9 could inhibit B16F1 growth and lung metastasis in vivo. ITGA9 may be a promising target of gene therapy for melanoma.