马齿苋脑苷A对人宫颈癌HeLa细胞凋亡和侵袭转移的影响

Effects of Portulacerebroside A on Apoptosis, Migration and Invasion of Human Cervical Cancer HeLa Cells

目的观察马齿苋脑苷脂类新化合物马齿苋脑苷A(portulacerebroside A,PCA)对人宫颈癌HeLa细胞凋亡和侵袭转移的影响。方法不同浓度PCA作用HeLa细胞不同时间后,采用MTT法观察药物对细胞活力的影响,流式细胞仪检测凋亡率,划痕试验观察细胞迁移百分率,基质胶法测定细胞黏附百分率,Transwell试验检测侵袭细胞数。结果 PCA显著抑制HeLa细胞活力,增加细胞凋亡率,降低细胞迁移百分率和黏附百分率,减少侵袭细胞数。结论 PCA显著诱导人宫颈癌HeLa细胞凋亡和抑制其侵袭迁移,提示其潜在抗宫颈癌作用。

Objective To examine whether portulacerebroside A(PCA), a novel cerebroside compound isolated from Portulaca oleracea L., influences the apoptosis, migration and invasion of human cervical cancer HeLa cells. Methods HeLa cells were treated with various concentrations of PCA. Cell viability was examined by MTT assay and the apoptosis induced by PCA was determined by flow cytometry. The scratch test, matrigel assay and Transwell test were performed respectively to estimate the capabilities of migration, adhesion and invasion of HeLa cells. Results PCA treatment significantly inhibited cell viability, increased apoptosis rate, decreased migration percentages and HeLa cells adhesion, and reduced invasion cells number. Conclusion PCA markedly induces the apoptosis and suppresses the migration and invasion of HeLa cells, suggesting its potential therapeutic effects on cervical cancer.