肺癌基因异常表达与化疗药物的相关性分析

Correlation of Aberrantly Expressed Lung Cancer Genes and Chemotherapy Drugs

目的通过肺癌基因表达谱分析与肺癌相关的化疗药物,探讨化疗药物与肺癌基因表达异常的相关性。方法首先通过统计学方法比较肺癌组织以及正常肺组织的表达谱数据,筛选出这两类组织的差异表达基因。明确肺癌组织基因的异常表达情况。然后通过差异表达基因与药物的关联分析,筛选肺癌组织中上调的差异表达基因所对应的化疗药物,最后筛选能够与多个化疗药物均相对应的基因,对这些基因进行网络调控分析,明确这些基因间的可能调控关系。结果筛选出在肺癌组织中表达上调的异常表达基因共397个,对应的常用化疗药物有6种,其中TOP2A、MAD2L1、BIRC5这三种基因对应着多个化疗药物,且MAD2L1可能通过P53直接或间接调控TOP2A和BIRC5的生物学功能。结论肺癌组织中表达上调的基因TOP2A,MAD2L1,BIRC5与肺癌的化疗效果可能有一定相关性,MAD2L1可能影响TOP2A,BIRC5的生物学功能。

Objective To explore the relationship between aberrantly expressed genes in lung cancer genes and after chemotherapy by gene expression profile. Methods In this article, expression profile of lung cancer tissue and normal lung tissue were compared to screen out the differentially expressed genes by statistical method. The aberrant expressed genes of lung cancer cells were clarified. Then the up-regulated aberrantly expressed genes were upregulated by the chemotherapy drugs were selected by association analysis. Finally, the genes which could correspond to many chemotherapy drugs were screened and analyzed by network regulation, to clarify the possible regulation relationship between these genes. Results Three hundred and ninety-seven up-regulated aberrantly expressed genes induced by 6 kinds of related chemotherapy drugs in lung cancer tissue were screened out. Among them, TOP2A,MAD2L1,BIRC5 corresponded to many chemotherapy drugs and MAD2L1 may control the biological function of TOP2A and BIRC5 through P53. Conclusion The up-regulated expression genes, TOP2A,MAD2L1 and BIRC5, may have correlation with the effect of chemotherapy of lung cancer. MAD2L1 may infl uence the biological function of TOP2A and BIRC5.