膀胱尿路上皮癌石蜡包埋组织中miR-20a的表达与复发的相关性分析

Correlation Analysis of miR-20a Expression and Relapse in Formalin-fixed Paraffinembedded Tissue of Urothelial Carcinoma of Bladder

目的探讨膀胱尿路上皮癌(膀胱癌)石蜡包埋组织中提取microRNA的可行性,分析miR-20a在膀胱癌石蜡包埋组织中的表达情况与临床病理特征及术后复发的关联性。方法荧光定量RT-PCR法检测50例新鲜膀胱癌组织和对应的石蜡包埋组织中miR-20a基因表达的相关性,分析不同年度间181例石蜡包埋膀胱癌组织中miR-20a基因的表达,并与患者术后复发进行相关性分析。结果石蜡包埋膀胱癌组织与新鲜冷冻组织中miR-20a的表达密切相关(r=0.792,P<0.001);不同年度间石蜡组织的miR-20a表达稳定,差异无统计学意义。miR-20a表达量与肿瘤临床特征明显相关,高表达患者其术后复发明显增高(P﹤0.05)。结论石蜡包埋膀胱癌组织与新鲜冷冻组织中miR-20a的表达有一致性,用石蜡组织提取microRNA是可行的,膀胱癌细胞中miR-20a的作用与其表达量相关,高表达是术后复发的独立因素。

Objective To investigate the feasibility of extracting microRNA from formalin-fixed paraffinembedded(FFPE) tissue of bladder urothelial carcinoma(UC), and analyze the correlation among miR-20a expression, clinicopathologic feature and the relapse. Methods The expression levels of miR-20a in freshly frozen(FF) and FFPE tissues from 50 cases of bladder UC were detected by quantitative real-time PCR. The expression levels of miR-20a in FFPE tissue from 181 cases of bladder UC and its correlation with the relapse in different years were analyzed. Results The expression of miR-20a were closely correlated in FFPE and FF tissue(r=0.792,P<0.001). The expression levels of miR-20a in FFPE tissue in different years were stable(P>0.05). The expression of miR-20a was closely correlated with clinicopathological features. The relapse rate of patients with high expression of miR-20a was significantly higher(P<0.05). Conclusion The expression levels of miR-20a in FF and FFPE tissue of bladder UC were consistency and reliable, hence, it is feasible to extract and study the microRNA in FFPE tissues. The role of miR-20a was correlated with its expression level, and a high expression level is the independent factor of postoperative relapse.