蜗牛多肽提取物对H_2O_2诱导的SH-SY5Y细胞脑源性神经营养因子表达的影响

Snail polypeptide extract effect on brain-derived neurotrophic factor expression in SH-SY5Y cell induced by hydrogen peroxide

目的研究蜗牛多肽提取物对H2O2诱导人神经母细胞瘤细胞(SH-SY5Y)氧化损伤的抑制作用。方法采用醋酸沉淀法提取蜗牛多肽,在H2O2诱导SH-SY5Y细胞损伤的基础上加入不同浓度的蜗牛多肽提取物,MTT法测定细胞存活率;细胞免疫化学技术和Western印迹技术分别检测脑源性神经营养因子(BDNF)的表达。结果 1.54 mmol/L H2O2可引起SH-SY5Y细胞存活率下降,BDNF的表达明显降低;39 mg/L和156 mg/L蜗牛多肽提取物对H2O2损伤的SH-SY5Y细胞的存活率有显著地提高作用(P<0.05),BDNF的表达也呈浓度依赖性升高(P<0.01)。结论蜗牛多肽提取物对H2O2损伤的SH-SY5Y细胞有保护作用,机制可能是通过上调BDNF的表达。

Objective To investigate protective effect of snail polypeptide extract( SPE) on hydrogen peroxide( H2O2) induced SH-SY5Y cells damage and its possible mechanism. Methods Snail polypeptide was extracted by acetic acid precipitation method. Under the condition of H2O2-induced SH-SY5Y cell damage,SPE with different concentrations as added. The viability of SH-SY5Y cells were measured by MTT. The expression of brain-derived neurotrophic factor( BDNF) was determined by using immunocytochemistry and western blotting,respectively. Results 1. 54 mmol / L H2O2significantly decreased the viability and BDNF expression in SH-SY5Y cells. After treatment with 39 mg / L and 156 mg / L SPE for 24 h,there was a concentration-dependent increase of the viability( P<0. 05) and BDNF expression in SH-SY5Y cells injured by H2O2( P<0. 01). Conclusions SPE could have protective effects on H2O2-inducing SH-SY5Y cells damage by increasing BDNF expression in the SH-SY5Y cells.