摘要
目的观察过表达外源岩藻糖基转移酶VII(FUT7)对人乳腺癌MDA-MB-231细胞黏附和迁移能力的影响。方法构建以FUT7为靶基因的真核表达载体pEGFP-C1-FUT7,转染人乳腺癌MDAMB-231细胞,采用RT-PCR检测FUT7 mRNA的表达;Western blot检测FUT7及其催化产物sLeX蛋白水平;细胞黏附实验及Transwell小室检测转染后细胞与HUVECs细胞黏附、迁移能力。结果转染细胞外源FUT7表达在mRNA水平和蛋白水平及sLeX含量均明显增加;转染的乳腺癌细胞黏附和迁移能力明显增强。结论过表达外源FUT7基因可增强人乳腺癌MDA-MB-231细胞黏附和迁移能力。
Objective To investigate the effect of exogenous overexpression of α1,3 FucT-Ⅶ(FUT7) on adhesion and migration of human breast cancer cell line MDA-MB-231. Methodes pEGFP-C1-FUT7 eukaryotic expression vector was constructed and transfected them into MDA-MB-231 cells. The expression level of FUT7 mRNA was examined by RT-PCR; Expression of FUT7 and catalysate sLeX protein were assayed by Western blot. Influence of exogenous FUT7 overexpression on adhesion and migration of MDAMB-231 cells to HUVECs was analyzed by adhesion assay and transwell assay. Results The exogenous expression levels of FUT7 mRNA,sLeX protein on cell surface were significantly increased in transfected MDA-MB-231 which had enhanced abilities of adhesion and cell migration. Conclusion Overexpression of exogenous FUT7 promoted the abilities of adhesion and migration of human breast cancer cell line MDAMB-231.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2014年第3期252-255,共4页
Cancer Research on Prevention and Treatment
基金
国家自然基金资助项目(81202084)
国家教育部重点项目资助课题(212047)
黑龙江省普通高等学校青年学术骨干项目(1253G067)
关键词
FUT7基因
转染
乳腺癌
黏附
迁移
FUT7 gene
Transfection
Breast Cancer
Adhesion
Migration
