WY14643对小鼠早期胚胎抗氧化损伤的影响

Effects of WY14643 on Antioxidative Damage of Early Mouse Embryos

旨在研究WY14643对小鼠早期胚胎发育的影响,探讨WY14643对早期胚胎抗氧化损伤的机理。通过H2O2诱导建立胚胎氧化损伤模型,添加WY14643对胚胎进行体外培养,DCHFDA测定胚胎内部H2O2含量,分别用超氧化物歧化酶(SOD)和微量丙二醛试剂盒测定胚胎不同发育阶段SOD活性和丙二醛MDA含量,并观察后续胚胎发育情况。结果表明:(1)不同浓度WY14643处理2h后,0.1μmol·L-1 WY14643处理组4-细胞率、囊胚率显著高于其他各组(P<0.05);(2)经0.1μmol·L-1 WY14643和H2O2联合处理后,其胚胎发育率显著高于单独H2O2处理组和对照组(P<0.05);(3)DCHFD荧光检测发现,WY14643添加组胚胎内部H2O2水平显著低于其他各组(P<0.05);(4)经SOD、MDA检测,发现添加WY14643胚胎内部各时期SOD水平显著高于其他各组(P<0.05),MDA水平显著低于其他各组(P<0.05)。综上表明,氧化应激对早期胚胎发育是不利的,WY14643通过增加抗氧化酶SOD活性、降低脂质损伤等途径减少胚胎内部ROS,增加抗氧化能力,促进胚胎在体外更好地发育。

The objective of this experiment was to study the effect of WY14643on the development of early embryos and to explore the mechanisms of antioxidative damage capacity in early mouse embryos.The model of oxidative damage was established by H2O2and added WY14643to the medium of invitroculture.H2O2level in embryos were measured by DCHFDA.SOD activities and MDA contents were determined by using superoxide dismutase and malondialchehyche assay kit,respectively.The results showed that:(1)after different concentrations of WY14643 processing to 2h,4-cell rate and blastocyst rate in the group of 0.1μmol·L-1 WY14643treatment was significantly higher than other groups(P<0.05);(2)after 0.1μmol·L-1 WY14643 with H2O2co-culture,the embryo development rate was significantly higher than only H2O2treatment group and control group(P<0.05).(3)through DCHFDA fluorescence detection,the H2O2 level of WY14643adding group was significantly lower than other groups(P<0.05).(4)from the SOD activities and MDA contents detection,we found that SOD level of adding WY14643group was significantly higher than other groups within each period(P<0.05)as opposite to the MDA level were significantly lower than other groups(P<0.05).Those results indicated that WY14643 could improve the development of embryo by increasing activity of SOD enzymes to reduce lipid damage and then decrease the H2O2level of the embryos in vitro culture.