SPF鸡感染禽白血病病毒A/B亚群后血清抗体与卵黄抗体的变化及其相关性

Dynamic of Yolk Antibody and Serum Antibody and Their Correlation in SPF Chickens Infected with Avian Leukosis Virus Subgroup A/B

拟研究以卵黄抗体替代血清抗体检测判定SPF鸡群禽白血病病毒(ALV)感染状态的可行性。在人工接种ALV-A/B后的40只SPF鸡刚开产时,从21只抗体阳性鸡及19只抗体阴性鸡分别采集血清和卵黄并一一对应,比较卵黄不同稀释度与血清中ALV-Ab抗体的阴阳性吻合率及ELISA检测S/P值相关系数。另外36只同批次不攻毒的SPF鸡单独饲养作为对照,76只鸡在25~34周龄期间,每隔3周分别采集1次血清,每周采集1次种蛋,共采集了304份血清样品及836份卵黄样品。所有血清和卵黄抗体用IDEXX的ALV-Ab抗体ELISA检测试剂盒检测。血清按试剂盒规定的1∶500稀释,卵黄按预试验结果选定稀释度稀释。同一只鸡同一时段采集的血清和卵黄样品,严格在同一次ELISA试验中检测。结果表明,相对于血清抗体,将卵黄做1∶300稀释时,假阳性和假阴性最少,确定1∶300为卵黄最适稀释度。在25~34周龄,836份卵黄抗体的检测判定结果与304份血清抗体检测结果吻合率达94.7%,即这些血清抗体阳性鸡同时期采集的卵黄抗体也全部阳性,血清阴性鸡的卵黄抗体也均为阴性。结果提示疫苗生产企业可通过检测SPF种蛋的卵黄抗体来判断SPF鸡场对ALV的洁净度。

The aim of this study was to study the feasibility of yolk antibody indicating the ALV infection status in SPF chickens instead of serum antibody.Eggs and serum samples were collected one to one correspondence from 40 23-weeks-old SPF chickens,which 21chickens were positive to ALV-Ab and 19chickens were negative.The egg yolk was diluted into 1∶150,1∶200,1∶300 respectively to compare the correlation of yolk antibody and serum antibody in the ELISA tests. 76SPF chickens with different ALV-Ab level were breed separately,eggs were collected once a week and serum was collected every three weeks from each chicken during the age of 25to 34 weeks,totally 836egg yolks and 304serum samples were collected.They were tested the antibody response to ALV-Ab by ELISA detection kits.The yolk antibody correlated well with serum antibody when the egg yolk was diluted at the 1:300dilution otherwise the false negative or false positives may arise.Antibody response to ALV-Ab in 836egg yolks correlated well with 304serum samples during the age of 25to 34weeks and the consistency rate is 94.7%.The yolk antibody can be as a good index to estimate the ALV infection in SPF chickens instead of serum antibody.