摘要
The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the conservative sequence D,D,D,QXXRW.One full-length cDNA of the cellulose synthase-like protein D(CslD) gene was cloned by reverse transcriptase(RT)-polymerase chain reaction(PCR) with 5’,3’ rapid amplification of cDNA ends(RACE) methods using degenerate primers designed from the homologous sequences of the CesA genes.A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence.Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids.The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity (over 71%) with the ClCslD genes from Populus tremuloides,Oryza sativa,and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants.
The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the conservative sequence D,D,D,QXXRW.One full-length cDNA of the cellulose synthase-like protein D(CslD) gene was cloned by reverse transcriptase(RT)-polymerase chain reaction(PCR) with 5’,3’ rapid amplification of cDNA ends(RACE) methods using degenerate primers designed from the homologous sequences of the CesA genes.A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence.Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids.The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity (over 71%) with the ClCslD genes from Populus tremuloides,Oryza sativa,and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants.
