大豆杂种后代过氧化物酶同工酶谱类型及其与亲本关系的分析

ANALYSIS OF TYPES AND RELATIONS OF PEROXIDASE IN SOYBEAN VARIETIES AND THEIR CROSSING PARENTS

采用聚丙烯酰胺凝胶垂直板电泳方法分析有亲缘关系的37个品种(包括15个品种的亲本来源)萌发6天的大豆子叶POD同工酶谱。结果表明,不同品种POD同工酶谱共显示5～10条酶带,分A、B、C三区。以B区酶带色度深浅及数目变化为分类标准,可分为四种酶型:PB_1型、PB_2型、PB_3型和PB_4型。根据此酶型分析了每一个组合的品种与其两亲本。其中6个品种呈现偏父本酶型,6个品种呈现新酶型,2个品种的酶型与父母相同,1个品种呈现偏母本酶型。同时测定了13个组合的36个品种的POD比活力,结果指出:后代品种POD比活力都低于或介于父母本之间,后代比双亲POD比活力有逐渐降低的趋势。

POD activities and POD bands in soybean seeding cotyledons were examined to study soybean parent - offspring POD relationships. Seeds with water absorption for 4 - 5 hours were studied POD activities. The results indicated that 5-10 bands of POD in different soybean culti-vars were demonstrated. Four enzyme types (PB1,PB2,PB3 and PB4)in B region were divided by the chromatism and numbers of bands. The enzyme types of six parent groups are as male- parent type, one parent groups as female parent, six parent groups appear new enzyme type,and en- zyme types of two parent groups like to parents. POD activities in 35 cultivars of 13 parent gro ps of soybean (G. max ) are lower than that of parents or middle of parents.