人类基因组miRNA转录调控区保守性DNA序列生物信息学分析

Bioinformatics Analysis of Homo Genome miRNA Conserved DNA Sequences in Transcriptional Regulation Regions

MicroRNA(miRNA)的表达调控方式一直是一个有争议的问题,为了研究miRNA潜在的转录调控特点,本文通过Sanger网站miRNA数据库获得人类miRNA的信息,并建立miRNA相关信息数据库,用MEME和Wordspy两个软件对其上游2000bp序列进行保守性分析,得到保守性的DNA序列(motif),用TESS软件分析保守性DNA序列,预测其转录因子结合情况。通过比较位于基因间、反义链和内含子中的三类不同miRNA转录调控区的保守性和自主转录能力的差异,结果发现位于基因间、反义链上的miRNA上游调控区的保守性比位于内含子的miRNA高,在miRNA的转录调控区存在RNA聚合酶Ⅱ类型的转录因子结合位点,miRNA还表现出自身独特的转录调控方式。通过分析,我们还得到了miRNA表达调控中一些重要的转录因子以及独特的调控序列。本研究结果为miRNA转录调控机制的进一步研究提供了理论依据。

The methode of microRNA(miRNA) transcriptional regulation always has been a disputations problem yet,in order to study the potential characteristic of miRNA regulation,in this paper,we acquired human miRNA information through miRNA database in Sanger website and established miRNA related information database.Both software of MEME and Wordspy were utilized to analyze the upstream 2000 bp sequences of the human miRNA,and then some conserved DNA sequences(motif) were obained,at last,we submitted all the conserved DNA sequences to software TESS to analyze the conserved DNA sequences and to predict the condition of their transcription factors binding.By comparing the conservation and the disparation of autonomous transcription ability of three kingds of different miRNAs which were located in intergenic,antisense and intron respectively.The results showed that the conservation of upstream regulation region of intergenic and antisen-miRNA was higher than that of intron-miRNA.And RNA polymerase Ⅱtranscription factor binding sites also presented in miRNA regulation regions.Additionally,miRNA also represented its own unique transcriptional regulation modes.We still scored some important transcription factors and unique regulating sequences in miRNA transcriptional regulation by analysis the data.All the results of this work would provide a theoretical basis for further study of miRNA transcriptional mechanism.