摘要
本研究采用根尖压片法,对3种不同肉质根皮色的萝卜种质进行染色体数目和核型分析,以获得准确的细胞遗传学信息。研究结果表明,Nau-dqp08,Nau-txbyw07和Nau-chhong108这3种不同皮色萝卜的染色体数目均为18,核型公式分别为2n=2×=18=14m+4sm,2n=2×=18=18m和2n=2×=18=16m+2sm,且核型都属于1A型;核不对称系数分别为59.48%、55.39%与58.08%;核型不对称性大小为Nau-dqp08>Nau-chhong108>Nau-txbyw07,其中Nau-txbyw07核型较为原始。本研究结果可为萝卜种质鉴定、遗传变异和亲缘关系分析提供细胞学依据。
In this research,we have analyzed the karyotype and chromosome number of three types of different tap-root skin color radish germplasms by using root-tip squashing method in order to obtain precise cyto-genetic data of radish.The results showed that chromosome number of Nau-dqp08,Nau-txbyw07 and Nau-chhong108 were all 18,and their karyotype formulas were 2n=2×=18=14m+4sm,2n=2×=18=18m and 2n=2×=18=16m+2sm,respectively,all of which belonging to '1A'according to Stebbins classification of karyotypic asymmetry,and karyotype asymmetry index of which were 59.48%,55.39% and 58.09%,respectively.Karyotype asymmetries ranked in the increasing order was Nau-dqp08>Nau-chhong108>Nau-txbyw07,and the karyotype of Nau-txbyw07 was more primitive among the total.The results of these experiments should be provided cytological information for radish germplasm identification,genetic variation and analysis of genetic relationship in radish.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2009年第6期1141-1145,共5页
Genomics and Applied Biology
基金
国家科技支撑计划课题(2008BADB1B03)
863计划(2008AA10Z150)
江苏省农业种质资源基因库项目[sx(2008)g09]共同资助
关键词
萝卜
染色体
核型分析
Radish(Raphanus sativus L.),Chromosome,Karyotype