摘要
The in situ forming biodegradable polymer scaffolds are important biomaterials for tissue engineering and drug delivery.Hydrogels derived from natural proteins and polysaccharides are ideal tissue engineering scaffolds since they resemble the extracellular matrices of the tissue comprising various amino acids and sugar based macromolecules.This work presented an injectable system from partially oxidized alginate and hydroxyapatite(HAP) nanocrystal for tissue engineering and drug delivery applications.In situ release of calcium cations from HAP nanocrystal was adopted through lowering the pH with slow hydrolysis of D-glucono-δ-lactone(GDL) and homogeneous alginate gels were formulated as scaffolds with defined dimensions.The gelation time could be controlled to be in 10-15 min.The SEM observations confirmed the porous 3D hydrogel structure with interconnected pores ranging from 20 to 300 μm and the HAP particles dispersed in the scaffolds uniformly.The potential applications such as tissue engineering scaffold and injectable drug delivery system were demonstrated by subcutaneous implant test in test rats.
The in situ forming biodegradable polymer scaffolds are important biomaterials for tissue engineering and drug delivery.Hydrogels derived from natural proteins and polysaccharides are ideal tissue engineering scaffolds since they resemble the extracellular matrices of the tissue comprising various amino acids and sugar based macromolecules.This work presented an injectable system from partially oxidized alginate and hydroxyapatite(HAP) nanocrystal for tissue engineering and drug delivery applications.In situ release of calcium cations from HAP nanocrystal was adopted through lowering the pH with slow hydrolysis of D-glucono-δ-lactone(GDL) and homogeneous alginate gels were formulated as scaffolds with defined dimensions.The gelation time could be controlled to be in 10-15 min.The SEM observations confirmed the porous 3D hydrogel structure with interconnected pores ranging from 20 to 300 μm and the HAP particles dispersed in the scaffolds uniformly.The potential applications such as tissue engineering scaffold and injectable drug delivery system were demonstrated by subcutaneous implant test in test rats.
基金
supported by the National High Technology Research and Development Program of China ("863" Project) (Grant No.2007AA091603)
the Intellectual Innovation Project of Guangdong University (Grant No.LYM08018)
