摘要
We have optimized the settings of evanescent wave imaging for the visualization of a protein adsorption layer.The enhancement of the evanescent wave at the interface brought by the incident angle,the polarized state of light beam as well as a gold layer is considered.In order to improve the image contrast of a protein monolayer in experiments,we have optimized three factors- the incident angle,the polarization of light beam,and the thickness of an introduced thin gold layer with a theoretical simulation.
We have optimized the settings of evanescent wave imaging for the visualization of a protein adsorption layer.The enhancement of the evanescent wave at the interface brought by the incident angle,the polarized state of light beam as well as a gold layer is considered.In order to improve the image contrast of a protein monolayer in experiments,we have optimized three factors- the incident angle,the polarization of light beam,and the thickness of an introduced thin gold layer with a theoretical simulation.
基金
the National High Technology Research and Development Program of China (Grant No. 02Z4)
the National Basic Research Program of China (Grant No. 2009CB320302) for their supports
