摘要
This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.
This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.
作者
ZHANG XiangMin1, LIU BaoHong1, ZHANG LiHua2, ZOU HanFa2, CAO Jing1, GAO MingXia1, TANG Jia1, LIU Yun1, YANG PengYuan1 & ZHANG YuKui2 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China
2Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
基金
support from the Major State Basie Research Development Program (Grant No. 2007CB914100)
the National Natural Science Foundation of China (Grant Nos. 20875016 & 20735005)
Shanghai Projects (Grant Nos. 08DZ2293601 & B109)
