摘要
A new protein molecularly imprinted polymer (MIP) was prepared with grafting polyvinyl alcohol as assistant recognition polymer chains (ARPCs). The ARPCs and acrylamide monomers were interpenetrated and then polymerized on the surface of macroporous acrylate adsorbent spheres. The template BSA was removed by treatment with 2.00 mol L-1 potassium chloride (KCl) solution and the adsorbed proteins were detected with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). 0.150, 0.500, and 2.00 mol L-1 KCl solutions were used as eluent to wash the adsorbed proteins. The SDS-PAGE results show that proteins washed out with 2.00 mol L-1KCl solution were from nonspecific adsorption of macroporous acrylate adsorbent spheres, and proteins washed out with 0.500 mol L-1KC1 solution were specific proteins imprinted by MIP resins. MIP resins with ARPCs had better recognition to the target proteins than that without ARPCs. The adsorption capacity of MIP resins immobilized ARPCs to the template BSA was about 80-100 μg g-1 when it was used for the adsorption of proteins mixture, and the specific adsorption of the target protein was obviously increased.
A new protein molecularly imprinted polymer (MIP) was prepared with grafting polyvinyl alcohol as assistant recognition polymer chains (ARPCs). The ARPCs and acrylamide monomers were interpenetrated and then polymerized on the surface of macroporous acrylate adsorbent spheres. The template BSA was removed by treatment with 2.00 mol L-1 potassium chloride (KCl) solution and the adsorbed proteins were detected with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). 0.150, 0.500, and 2.00 mol L-1 KCl solutions were used as eluent to wash the adsorbed proteins. The SDS-PAGE results show that proteins washed out with 2.00 mol L-1KCl solution were from nonspecific adsorption of macroporous acrylate adsorbent spheres, and proteins washed out with 0.500 mol L-1KC1 solution were specific proteins imprinted by MIP resins. MIP resins with ARPCs had better recognition to the target proteins than that without ARPCs. The adsorption capacity of MIP resins immobilized ARPCs to the template BSA was about 80-100 μg g-1 when it was used for the adsorption of proteins mixture, and the specific adsorption of the target protein was obviously increased.
作者
GUO MinJie1, GAO Ting1, FAN Zhi1, YAO JingXia1, XIA JianJun2 & MI HuaiFeng2 1Department of Chemistry, College of Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
2Key Laboratory of Functional Polymer Materials, Ministry of Education
Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China
基金
supported by the National Natural Science Foundation of China (Grant Nos. 20704031 and 20674040)
Science Fund of Tianjin Education Committee (Grant No. 20060515)
Natural Science Foundation of Tianjin (Grant No. 09JCYBJC06300)
Science Fund of Tianjin University of Science and Technology (Grant No. 20060420)
