摘要
Seryl-Histidine dipeptide(Ser-His) has been previously reported to be capable of cleaving DNAs and carboxyl esters,as well as proteins.The protein cleavage mechanism has not been addressed yet.As an initial step of protein cleavage activity,the non-covalent binding affinity of Ser-His for proteins is a crucial prerequisite.In this work,we took cyclophilin A(CyPA) as a substrate protein,and evaluated the non-covalent interaction between CyPA and Ser-His using a combination of NMR spectroscopy and molecular modeling approach.Two independent Ser-His binding sites on CyPA were detected using 15N-1H heteronuclear single-quantum coherence(HSQC) spectra.Each binding site binds one Ser-His molecule.Dissociation constants,Kd1 and Kd2,were estimated to be 2.07 and 6.66 mmol/L,respectively,indicative of the weak non-covalent interaction between Ser-His and CyPA.Based on molecular modeling results,we suggest that both the α-amino and the side chain hydroxyl group of Ser-His are crucial for the non-covalent interaction between Ser-His and CyPA.This work sheds light on the molecular mechanism of Ser-His and its analogues cleaving proteins.
Seryl-Histidine dipeptide(Ser-His) has been previously reported to be capable of cleaving DNAs and carboxyl esters,as well as proteins.The protein cleavage mechanism has not been addressed yet.As an initial step of protein cleavage activity,the non-covalent binding affinity of Ser-His for proteins is a crucial prerequisite.In this work,we took cyclophilin A(CyPA) as a substrate protein,and evaluated the non-covalent interaction between CyPA and Ser-His using a combination of NMR spectroscopy and molecular modeling approach.Two independent Ser-His binding sites on CyPA were detected using 15N-1H heteronuclear single-quantum coherence(HSQC) spectra.Each binding site binds one Ser-His molecule.Dissociation constants,Kd1 and Kd2,were estimated to be 2.07 and 6.66 mmol/L,respectively,indicative of the weak non-covalent interaction between Ser-His and CyPA.Based on molecular modeling results,we suggest that both the α-amino and the side chain hydroxyl group of Ser-His are crucial for the non-covalent interaction between Ser-His and CyPA.This work sheds light on the molecular mechanism of Ser-His and its analogues cleaving proteins.
作者
LIU Yan1,SHI YanHong2,LIU XiaoXia1,LIN MingKun1,LIN DongHai1,2 & ZHAO YuFen1,3 1Key Laboratory for Chemical Biology of Fujian Province,Department of Chemical Biology,Department of Chemistry,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China 2Shanghai Institute of Materia Medica,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China 3Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology,Ministry of Education,Department of Chemistry,School of Life Sciences and Engineering,Tsinghua University,Beijing 100084,China
基金
supported by grants from the National Natural Science Foundation of China ( 20732004, 30730026, 20805037)
the Ministry of Science and Technology of China (2007CB914304)
