乙型肝炎病毒核酸载量与血清学标志物相关性的研究

Relativity analysis between hepatitis B virus DNA and serological markers

目的分析HBVDNA载量与血清学标志物的相关性。方法应用乙型肝炎病毒核酸试剂定量检测468份献血员样本HBVDNA含量,并分别定性或定量检测HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc及抗-HBcIgM血清学标志物,计算不同病毒核酸水平样本中5个乙肝血清学标志物阳性率及HBsAg、抗-HBs水平的分布情况。结果HBVDNA阴性样本中HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc阳性率分别为12.8%、39.8%、0、21.1%、49.6%,而HBVDNA阳性样本中HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc血清学标志物阳性率分别为91.3%、0.7%、20.9%、65.0%、88.6%,血清学指标在不同HBVDNA水平的阳性率差异具有统计学意义(χ2=197.4,P<0.01)。HBVDNA载量与HBsAg水平成正相关性,与抗-HBs水平成负相关性。结论低水平的HBsAg在不同HBVDNA载量水平的样本均有分布,HBsAg检测能力需要进一步提高,另外在我国人群中存在低乙型肝炎病毒核酸水平携带者,乙型肝炎病毒核酸可以弥补血清学检测试剂的不足。

Objective To study the relationship between the copies of HBV and serologic makers. Mothods Detect HBV DNA and serum HBsAg, Anti-HBs, anti-HBc, HBeAg, anti-HBe and anti-HBc IgM of 468 samples. To c, omtpare the positive rate of different serum markers in different HBV DNA levels, and analyses the relationship between HBV DNA levels and quantitative HBsAg, anti-HBs levels. Results In HBV DNA-negative samples, the positive rote of HBsAg, anti-HBs, anti-HBc, HBeAg, anti-HBe is 12.8%, 11.3%, 0, 21.1%, 49.6% separately. In HBV DNA-positive samples, that is 9t.3%,0.7% ,20.9% ,65.0% ,88.6%. There are obviously significant difference among serological markers in different HBV DNA levels (χ2=197.4, P<0.01). The results show the positive correlation between HBV DNA levels and quantitative HBsAg and the negative correlation between HBV DNA levels and quantitative anti-HBs. The positive rate of HBeAg(χ2=39.6, P<0.01), anti-HBe(χ2=27.0, P<0.01), anti-HBc(χ2= 13.6, P<0.01) in HBV DNA-positive samples all are higher than that of in HBV DNA-negative samples. Conclusion The lower-level of HBsAg exits in HBV DNA-positive samples regardless of DNA levels. The detecting potency for HBsAg should be improved further. Moreover there are carriers with lower HBV DNA level in China. The combination of HBV serological assays with HBV DNA NAT will increase blood safety.