EB病毒感染诱导Akata的c-fos及BZLF1基因的短暂表达

Epstein-Barr virus infection induces rapid c-fos and BZLF1 transient expression in EBV-negative Burkitt’s lymphoma-derived Akata

目的研究EB病毒感染对EB病毒阴性的Burkitt’s淋巴瘤细胞株Akata的B细胞早早期癌前基因c-fos及病毒激活的关键转录因子BZLF1的表达的影响。方法应用EB病毒(来源于EB病毒阳性Akata∕GFP.EBV.c18)感染EB病毒阴性的Burkitt’s淋巴瘤细胞株Akata,使用RT-PCR和蛋白质印迹技术分别检测EB病毒感染不同时间后细胞c-fos和EB病毒的BZLF1的表达。结果EB病毒感染Akata后30min到3h期间c-fos的表达明显增加,随后恢复正常;EB病毒感染后1.5h到48h的BZLF1的短暂表达,随后消失;而且EB病毒感染后6h到12hBZLF1蛋白也有短暂表达,随后消失。结论EB病毒感染能诱导Akata的B细胞c-fos及随后的BZLF1的短暂表达。

Objective To investigate the influence on expression of early gene c-fos and the BZLF1 transient in EBV-negative Burkitt' s lymphoma-derived Akata cells infected by EB virus. Methods EBV-negative Burkitt' s lymphoma-derived Akata cells were infected by EBV from Akata\GFEEBV.c18. Moreover c-fos and BZLF1 expression postinfection were detected by RT-PCR or Western blot. Results The rapid increase of c-los expression from 30 min to 3 h post-infection and BZLF1 or BZLF1 protein transient expression from 1.5 h to 48 h or from 6 h to 12 h(there after disappeared) were detected by RT-PCR or Western blot. Conclusion These results indicated that EBV infection could induce c-los and then BZLF1 transient expression in Akata cells.