PEG对大豆(Glycine max)下胚轴脂氧合酶(LOX)活性及蛋白质含量的影响

EDDECTS OF PEG PRETREATMENT ON THE LOX ACTIVITY AND PROTEIN CONTENT IN SOYBEAN HYPOCOTYL

众所周知,大豆脂氧合酶(LOX)活性与生长发育密切相关。业已证实,PEG(聚乙二醇)处理大豆种子可促进其下胚轴中蛋白质的合成。本实验结果表明:PEG处理大豆种子后,其下胚轴中的脂氧合酶活性与未经PEG处理的对照相比显著降低。而蛋白质的含量却高于对照。因此推测,下胚轴中引起酶促脂质过氧化作用的主要酶—脂氧合酶活性的降低,与蛋白质总含量的升高密切相关。 PEG高渗溶液具有引发种子的效果,一方面是由于PEG处理可以改善种子的膜透性使损伤得到恢复,因而提高种子活力。另一方面是由于PEG处理种子会引起ATP的迅速合成与消耗,最终使新陈代谢作用增强的缘故。对植物体内LOX活性与蛋白质含量间关系的研究报道不多,且争论颇大。本文在PEG处理种子的前提下,首次探讨了大豆下胚轴脂氧合酶活性与蛋白质含量之间的关系,为LOX生理功能和PEG引发种子机制的进一步研究提供了依据。

It is well known that the Liopxygenases in soybean are closely related to growth and development. It has been proven that the pretreatment of PEG on soybean seeds may accelerate the synthesis of protein in soybean hypocotyl. Our results indicated, after the PEG pretreatment on soybean seeds, the Lipoxygenase in soybean hypocotyl would decrease, (compared with the untreated). However, the protein content would increase. So, we speculate that the decline of the Liopxygenase activity was closely related to the raise of the protien content in soybean hypocotyl.