DL-α-硫辛酸对大鼠实验性糖性白内障抑制作用的体外研究

Effects of DL-α-Lipoic acid on glutathione concentration in rat lens in model sugar cataractogenesis

目的 本研究观察高浓度葡萄糖对体外培养的大鼠晶状体的损害 ,以及抗氧化剂DL α 硫辛酸 (LA)的保护作用。方法 雄性、7周龄Wistar大鼠 ,CO2 处死后在手术显微镜下 ,连同囊膜完整摘出晶状体 ,放入 2 4孔组织培养板中培养。预培养 4 8h过程中 ,剔除受损伤的晶状体。然后晶状体或者被放入正常浓度 (5 .5 6mmol·L-1)葡萄糖培养液中培养 ,作为对照组 ,或者被放入高浓度 (5 5 .6mmol·L-1)葡萄糖培养液中培养 ,作为实验组。每组再分成 0、0 .5、1.0和 2 .0mmol·L-1LA亚组 ,每个亚组含有 6个晶状体。用照相方法记录培养过程中大鼠晶状体的形态改变 ,培养 5d后测量晶状体中谷胱甘肽的水平。结果 (1)对照组培养 5d晶状体未见明显混浊 ,其中 0 .5和 1.0mmol·L-1LA都能保持晶状体透明 ,但在 2 .0mmol·L-1LA中 ,从赤道部开始 ,晶状体表面出现不规则黄色混浊。实验组培养 2 4～ 4 8h ,晶状体开始膨胀 ,且在赤道部发生轻微的混浊 (白内障 ) ;培养5d ,晶状体膨胀 ,皮质内可见片状白色混浊 ,赤道部出现致密 ,黄白色环状混浊。在0 5mmol·L-1LA中 ,晶状体虽膨胀 ,但保持透明 ,在 1 0和 2 0mmol·L-1LA中 ,晶状体膨胀同时 ,赤道部出现不同程度的环状混浊 ,其中 2 0mmol·L-1LA混浊程度最重 ;(2 )在正常浓度葡?

Objective To probe into the effects of glucose on the culture rats' lenses and the response to the antioxidant DL-α-Lipoic acid (LA).Methods Lenses with intact capsules were dissected from rats' eyes (Wistar, 7 weeks old, male) following CO 2 anaesthesia.After dissection, each lens was transferred into a 24-well tissue culture plate in MEM for pre-incubating 48 hours, and any opaque lens was discarded. Forty-eight lenses were incubated in MEM containing 55.6mmol·L -1 glucose or containing 5.56mmol·L -1 glucose, either without LA, or with 0.5,1.0 and 2.0mmol·L -1LA addition. During the incubation, the lenses were documented by photography. After 5 days incubation, lens glutathione was tested.Results MEM with 5.56mmol·L -1glucose induced lens swelling up, and a cortical opaque. With 0.5mmol·L -1LA, the lenses kept transparent.But with 1.0, 2.0mmol·L -1LA, the lenses developed swelling and opacity, especially with 2.0mmol·L -1LA.Incubated in 5.56mmol·L -1 glucose, the lens glutathione concentration was(1 573±33)μmol·L -1(n=6).In 55.6mmol·L -1 glucose that was reduced to be(813±201)μmol·L -1(n=6), decreased about 48.3%. With 0.5mmol·L -1LA addition,there was 1.5 times higher than that of without LA addition.With 1.0mmol·L -1LA,there was 1.6 times higher and 0.8 times higher with 2.0mmol·L -1LA.Conclusion LA treatment protected against glucose-induced lens changes, and 0.5mmol·L -1was the efficient concentration;with maintaining glutathione concentration 1.0mmol·L -1 was the most efficient concentration of LA.