唾液腺腺样囊性癌患者体内抑癌基因PTEN和血管内皮生长因子的表达及相关性研究

Relationship between expression of PTEN and VEGF in salivary adenoid cystic carcinoma

目的研究抑癌基因PTEN、血管内皮生长因子(VEGF)在唾液腺腺样囊性癌(salivary adenoid cysticcarcinoma,SACC)患者体内的表达及相关性。方法选取滕州市中心人民医院病理科存档处保存的2000-2007年涎腺手术切除后的石蜡标本,其中SACC30例,多形性腺瘤(PA)26例,以正常涎腺组织10例为对照。应用免疫组化链霉菌抗生物素蛋白-过氧化酶连接(SP)法检测PTEN、VEGF在SACC、PA及正常涎腺组织中的蛋白表达。结果SACC中PTEN阳性表达率(43.33%)显著低于PA(92.30%)和正常涎腺组织(100%)(P<0.01),SACC中VEGF阳性表达率(86.67%)显著高于PA(23.09%)和正常涎腺组织(0)(P<0.01);PTEN与VEGF蛋白表达呈负相关(r=-0.40,P<0.05)。结论PTEN与VEGF在SACC的发生发展中可能起重要作用,PTEN蛋白表达的降低或缺失可能增加VEGF的表达,从而促进肿瘤的血管生成,导致肿瘤恶性进展。

Objective To investigate the relationship of the protein expression of PTEN and VEGF in salivary adenoid cystic carcinoma(SACC)Methods Using immunohistochemical SP method,the protein expression of PTEN and VEGF were examined in 30 SACC and 26 pleomorphic adenoma(PA)and 10 normal salivary samples.Results The positive rates of PTEN protein in SACC(43.33%)were significantly lower than those of PA(92.30%)and normal salivary samples(100%).The positive rates of VEGF protein in SACC(86.67%)were significantly higher than those of PA(23.09%)and normal salivary samples(0%),respectively.There was a negative correlation between PTEN and VEGF expression(r=-0.40,P<0.05).Conclusion Inactivation of PTEN gene in SACC may promote angiogenesis through increase of VEGF expression,and thus induce tumor progression.