运动发酵单胞菌乙醇发酵条件的优化

Optimization of ethanol fermentation by Zymomonas mibilis

通过对运动发酵单胞菌(Zymomonas mobilis)乙醇发酵条件和培养基成分分别进行单因子优化和正交优化,确定运动发酵单胞菌Z1乙醇发酵最佳葡萄糖含量为10%~15%,最适接种量为发酵培养基体积的1%,最佳发酵温度为30~35℃,培养基最适pH为6.0~9.0;其发酵培养基最佳基础成分为:MgSO40.01%,(NH4)2SO40.18%,KH2PO40.18%,酵母膏0.9%;在这4种成分中酵母膏对乙醇产率的影响最大。

After the optimization of single-factor experiments and orthogonal optimization of medium components by Z.mobilis Z1,the optimal ethanol fermentation conditions are obtained as follows:glucose content of 10%～15%,pH=6.0-9.0,the amount of inoculum as 1%;the best basis for fermentation medium components are:MgSO4 0.01%,(NH4)2SO4 0.18%,KH2PO4 0.18%,yeastextract 0.9%;and in these four components yeastextract affects the ethanol yield the most.