沼泽红假单胞菌与小球藻USTB-01的共培养研究

Study on co-culture between Rhodopseudomonas palustris and Chlorella USTB-01

围绕沼泽红假单胞菌和小球藻USTB-01共培养的优化控制条件进行了研究。采用光合细菌培养基,在沼泽红假单胞菌和小球藻USTB-01共培养条件下,可以明显促进沼泽红假单胞菌的生长,但小球藻USTB-01的生长却没有明显的提高。进一步研究发现在培养基中加入葡萄糖后可以明显促进小球藻USTB-01的生长,但高浓度4 g/L的葡萄糖会引起体系pH下降,不利于沼泽红假单胞菌的生长。在初始加入1 g/L的葡萄糖时,共培养体系中pH先下降后上升,能同时促进小球藻USTB-01和沼泽红假单胞菌的生长。

Optimal control for the co-culture between Rhodopseudomonas palustris and Chlorella USTB-01 is studied.The results show that the co-culture between R.palustris and Chlorella USTB-01 can significantly promote the growth of R.palustris,but the growth of Chlorella USTB-01 is not improved using the medium of photo-synthetic bacteria.The growth of Chlorella USTB-01 can be promoted by adding glucose in the medium.The initial glucose of 4 g/L might soon decrease pH below 7.0,which might inhibit the growth of R.palustris.However the initial glucose of 1 g/L might firstly decline pH and then increase pH of culture medium,and the growth of both R.palustris and Chlorella USTB-01 can be improved.