冠心康片总黄酮含量的测定

Determination of Total Flavonoids Contents from Guanxinkang Tablet

目的采用分光光度法测定冠心康片中总黄酮的含量。方法以芦丁为标准品建立标准曲线,检测波长为510nm。结果回归方程为C=-0.00142+0.8558A,在0.0751～0.6004mg内呈良好线性关系(r=0.9999),平均加样回收率为99.42%,RSD为1.64%(n=5)。不同批号冠心康片每片总黄酮平均含量为0.3631mg。结论该方法简便、准确,适用于冠心康片的质量分析检验。

OBJECTIVE To determine the contents of total flavonoids in Guanxinkang tablet by spectrophotometry.METHODS Taking rutin as the control to make standard curve,the detective wavelength was 510 nm.RESULTS Regression equation was C=-0.001 42+0.855 8A(r=0.999 9).The linear concentration was in the range of 0.075 1-0.600 4 mg for rutin.The average recovery was 99.42%,RSD was 1.64%(n=5).The average contents of total flavonoids from Guanxinkang tablet with different numbers was 0.363 1 mg.CONCLUSION The method is simple and accurate,it can be used for quality control of Guanxinkang tablet.