玉米秸秆生物产氢菌的分离培养及鉴定研究

Isolation and characterization of the bacteria in biohydrogen production system

以牛粪为菌种来源,利用玉米秸秆进行厌氧发酵产氢实验,在产氢效果达160mL/h,秸秆产氢能力225mL/g的最佳状态下,从实验中提取发酵菌液进行细菌的分离培养,并对产氢效果最好的R-3菌种进行生理生化试验研究和初步鉴定,确定产氢细菌为Clostridiumsp.Fanp2。

Corn stalk is used for bio-hydrogen production by anaerobic fermentation from cow dung.Under the optimal conditions of hydrogen production of 160 mL/h and straw hydrogen production capacity of 225 mL/g,the fermented bacterial liquid is extracted for bacteria isolation and culture,and the R-3 bacteria with best hydrogen production capacity is chosen to conduct preliminary identification and physiological and biochemical investigation.The type of hydrogen-producing bacteria is identified as Clostridium sp.Fanp2.