表油菜素内酯对苦参愈伤组织生长的影响

Effect of Epibrassinolide on Callus Growth of Sophora flavescens in Tissue Culture

在苦参愈伤组织生长的最佳培养基:改良MS+TDZ 1.0 mg/L+2,4-D 0.5 mg/L+Cys20 mg/L上分别添加表油菜素内酯(epibrassinolide,epi-BR)0、10^(-7)、10^(-5)、10^(-3)、10^(-1)mg/L。结果表明,愈伤组织鲜重随epi-BR浓度升高而升高,呈正相关,而干重则在10^(-3)mg/L epi-BR浓度下达到最高,之后随epi-BR浓度升高呈下降趋势。

Epibrassinolide was investigated during callus growth of Sophora flavescens.The callus were cultured on an optimized MS medium,supplemented with series concentration of epibrassinolide(0,10^(-7),10^(-5),10^(-3),10^(-1) mg/L respectively).The results indicated epibrassinolide significantly influenced the callus growth.The fresh weight of callus increased with increasing the concentration of epibrassinolide in the medium.The dry weight increase of callus showed different results,which got highest yield when the medium contained 10^(-3) mg/L epibrassinolide,but it decreased when epibrassinolide concentration was over 10^(-3) mg/L.