猪肉中13种磺胺类药物多残留检测超高效液相-串联质谱法

Simultaneous Determination of 13 Sulfonamides in Porcine Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了用超高效液相-串联质谱仪检测猪肉中种磺胺多残留的方法。采用乙腈提取,正己烷去脂,碱性氧化铝柱净化后,用超高效液相-串联质谱仪进行定性及定量分析。方法最低检出限为0.25μg/kg。方法灵敏、准确,可实现多种磺胺激素同时进行定性及定量分析。

A ultra performance liquid chromatography-tandem mass spectrometric(UPLC/MS/MS) method was developed for simultaneous determination of 13 sulfonamides in the porcine meat.Components were extracted from homogenized sample with acetonitrile,defatted by n-hexane and further extracted by Alumia B.The limit of determination(LOD) was 0.25 μg/kg.The method was proved to be satisfactory in precision,securacy and sensitivity for multi-residues determination.