饲用酶添加剂预混料中酸性蛋白酶活力测定方法研究

Research on the Determination of Acidic Protease Activity in Feed Premixed with Enzyme Additive

本文就《工业酶制剂通用试验方法》测定饲用酶添加剂预混料中酸性蛋白酶的活力进行了探讨,并就试验方法的适用性以及影响酶活力测定的因素进行了试验论证。结果表明:《工业酶制剂通用试验方法》回收率低,不适合于饲用酶添加剂预混料中酸性蛋白酶活力的测定;其他酶的存在不会影响酸性蛋白酶的活力测定;载体对酸性蛋白酶有较强的吸附力,结果导致《工业酶制剂通用试验方法》回收率偏低;《工业酶制剂通用试验方法》改进方法避免了载体吸附造成的回收率低的因素,回收率由原方法的70%左右提高到了93%以上。

This paper focused on discussion of using the method in 'General determination of industrial enzymes' to detect acidic protease activity of enzyme additive in feed premixed with enzyme additive.The applicability of test methods and the factors that affect the determination of enzyme activity were determined in this paper.The results showed that: the method in 'General determination of industrial enzymes' has a low recovery rate,it is not suitable to determine the acid protease activity of enzyme additive in feed premixed with enzyme additive;The presence of other enzymes will not affect the determination of acid protease activity;The absorption ability of carrier to acid protease is so strong that it causes the lower recovery in original method;In this research,the method was improved to prevent the low recovery caused by absorption of the carrier,the recovery is enhanced from 70%(the recovery of the original method) to more than 93%.