高效液相色谱法测定灵芝多糖含量

Determination of Ganoderma Lucidum Polysaccharide by Reversed-phase High Performance Liquid Chromatography

建立了高效液相色谱法测定灵芝多糖含量的方法,采用糖专用分析柱,以104分子量的葡聚糖为标准,流动相为水,流速0.6 mL/min,检测器为示差检测器,柱温为70℃,检测限为0.03 g/L,线性方程为Y=109 779.6X-903.3相关系数r=0.999 9,方法回收率为98%~104%,相对标准偏差为RSD%=0.12(n=6)。该方法简易、稳定,适合灵芝多糖的测定。

A reversed-phase high performance liquid chromatographic method(HPLC) for the determination of ganoderma lucidum polysaccharide was developed,using SUGUR PARK-Ⅰ(6.5×300 mm) as the analysis column,glucan 10 000 as standard,water as mobile phase at a flow rate of 0.6 mL/min.The differential refractometer detector was used and the column temperature was 70℃.The limit of detection was 0.03 g/L.The linear regression equation was Y=109 779.6X-903.3,the correlation coefficient r=0.999 9.The method recoveries ring from 98% to 104%.Relative standard deviation was 0.12%(n=6).The method is simply,stable,suitable for the determination of ganoderma lucidum polysaccharide.