青花菜游离小孢子培养体系的优化研究

Study on optimizing the system of isolated microspore culture of broccoli(Brassica oleracea var. italica)

对5个青花菜材料进行小孢子培养,有3个成功诱导出胚状体.选择出胚率较高的材料进行青花菜小孢子培养的优化研究,结果表明:材料冬性的强弱可能对小孢子胚状体的获得产生影响;当花蕾大小在3.6～4.5mm之间时,多数小孢子处于单核晚期和双核期,适宜小孢子培养;32.5℃热击处理1d,25℃暗培养10～12d后,40～60r/min的摇床恒温振荡培养1周左右能显著提高胚状体的质量.

Isolated microspores of five different broccoli materials were cultivated,embryoids had been obtained from three varieties.Among these materials,B08004 and B08008 had a higher inducement ratio.We chose them as the experimental materials for the optimization of isolated microspore culture.The results showed that,the winterness might effect the frequency of embryogenesis;The optimum stage for the microspore cultivation was from uninucleate anaphase to dikaryolization phase,and at this time the length of the buds were between 3.6 to 4.5 mm;The heat-shocked treatment at temperature 32.5℃,static culturing in dark condition at 25℃ 10—12d,and shake-culturing at 40～60r/min for about one week could obviously improve the quality of embryoids.