高效液相色谱法测定伤益凝胶中人参皂苷Rg_1的含量

Determination of ginsenoside Rg_1 in Shangyi Gel by HPLC

建立伤益凝胶中人参皂苷Rg1的含量测定方法.采用高效液相色谱法测定伤益凝胶中人参皂苷Rg1的含量.人参皂苷Rg1进样量在0.1212～12.120μg(R=0.99999)范围内与峰面积呈良好的线性关系,平均回收率为97.8%,RSD为2.31%(n=6).该方法灵敏、准确、重现性好.可作为伤益凝胶的含量测定方法.

To establish methods for the determination of ginsenoside Rg1 in Shangyi Gel.HPLC was adopted in the determination of ginsenoside Rg1 in Shangyi Gel.There were good liner relationships between the peak areas and the sample contens injected at the ranges of 0.1212—12.120μg (R=0.99999)for ginsenoside Rg1.The average recovery rates of ginsenoside Rg1 were 97.8%,RSD was 2.31%(n=6).The methods are simple,rapid and accurate.They can be used for quality control of Shangyi Gel.