摘要
目的:观察NG701对泡沫细胞形成及CD36mRNA表达的影响。方法:利用大鼠腹腔提取的巨噬细胞与氧化低密度脂蛋白共培养得到巨噬细胞源性泡沫细胞,加入不同浓度的NG701,观察泡沫细胞内胆固醇含量的变化,并利用RT-PCR法对CD36mRNA表达情况进行检测。结果:NG701能够显著减少泡沫细胞内总胆固醇和胆固醇酯的含量,降低CE/TC的值。并剂量依赖性的降低CD36mRNA表达。结论:NG701能通过减少CD36表达抑制巨噬细胞源性泡沫细胞的形成。
Objective: To investigate the effect of NG701 on the formation of foam cell and the expression of CD36 mRNA. Methods: Macrophages were incubated with ox-LDL to establish the foam cell model. Different concentration of NG701 were given for intervention. The cellular lipid accumulation was examined by oil red staining. The cellular contents of total cholesterol (TC) and cholesterol ester (CE) were detected by enzymic methods. The levels of CD36 mRNA were determined by RT-PCR. Results: NG701 could significantly decrease the content of TC, CE, and CE/TC in foam cell in middle and high dose groups. RT-PCR assays showed that the expression of CD36 mRNA was down-regulated in a dose-dependent manner. Conclusion: NG701 could inhibit the formation of foam cell via down-regulating CD36 mRNA expression.
出处
《药物评价研究》
CAS
2009年第2期84-87,共4页
Drug Evaluation Research
