HPLC法测定参茸健脾胶囊中10-羟基-2-癸烯酸

Determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula by HPLC

目的:建立参茸健脾胶囊中10-羟基-2-癸烯酸的HPLC测定方法。方法:采用Agilent 1100 DAD高效液相色谱仪,Agilent Extend C18色谱柱(250mm×4.6mm,5μm),乙腈?0.1%磷酸(23∶77)为流动相,检测波长为207nm,流速1mL/min。结果:10-羟基-2-癸烯酸在0.0354～0.4243μg范围内线性关系良好(r=0.9995),平均回收率为100.6%,RSD=1.9%。结论:该法简便、快速、准确,适用于参茸健脾胶囊中10-羟基-2-癸烯酸的定量测定,有利于控制参茸健脾胶囊的质量。

Objective: To establish an HPLC method for simulataneous determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula. Methods: The HPLC was conducted on discovery Agilent Extend C18 column (250 mm × 4.6 mm, 5 μm). with acetonitrile-0.1% phosphoric acid solution (23:77) as the mobile phase. The detection wavelength was 207 nm, and flow rate was 1 mL/min. Results: There were a good linear relationship of 10-hydroxy-2-decenoic acid within 0.035 4— 0.424 3 μg (r = 0.999 5). The average recovery rate was 100.6%, RSD was 1.9%. Conclusion: The method is simple, rapid and accurate for the determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula, and it is expected for the quality control of Shenrong Jianpi Capsula.