IL-12B mRNA在儿童及青少年Hp相关性胃十二指肠疾病中的表达

Expression of IL-12B mRNA in stomach and duodenal diseases infected with Helicobacter pylori in children and adolescent

目的研究白细胞介素12B(IL-12B)mRNA在儿童及青少年幽门螺杆菌(Hp)相关性胃十二指肠疾病胃黏膜中的表达。方法采用RT-PCR方法检测胃黏膜IL-12B mRNA表达。血清Hp-IgG抗体检测、PCR、尿素酶实验及组织学检查作为确定Hp感染状态的依据。结果儿童及青少年Hp感染者胃窦黏膜IL-12B mRNA阳性表达率、相对表达水平均较无Hp感染者高。IL-12B mRNA相对表达水平与胃窦黏膜慢性炎症程度呈正相关。结论儿童及青少年Hp相关性胃十二指肠疾病胃窦黏膜IL-12B mRNA表达上调可能促进Th1细胞免疫反应从而增加黏膜破坏。

Objective To study the expression of IL-12B mRNA in stomach and duodenal diseases infected with Helicobacter pylori(H.pylori) in children and adolescent.Methods IL-12B mRNA expression was measured with RT-PCR technology.Serology,PCR,urease test and histology were used to check H.pylori infection.Results In children and adolescent,the positive rate and level of IL-12B mRNA expression were significantly higher in H.pylori infected patients than in uninfected ones.The level of IL-12B mRNA expression was significantly positive correlated with the degree of antrum chronic inflammation.Conclusions Increased IL-12B mRNA expression of antral mucosa in H.pylori infected children and adolescent may promote Th1 cell mediated immunoresponses,which associated with increased mucosal damage.