不同环境条件下偶氮染料酸性大红GR的生物降解性能

Biodegradation Potential of Azo Dye C.I. Acid Red 73 under Different Environmental Conditions

为了考察不同环境条件下偶氮染料酸性大红GR生物降解性能,采用已驯化的混合菌群作为接种液进行偶氮染料酸性大红GR脱色试验。结果表明,微氧条件下(静置敞口培养),偶氮染料酸性大红GR脱色效果最佳,染料脱色主要发生在菌体的"对数生长期"。混合菌群对pH、温度适应范围较广,pH为3.32~9.18,温度在20℃~37℃范围内均可以获得较好脱色效果,脱色率均在80%以上。微氧条件下酸性大红GR降解历程表明,偶氮基整个共轭系统被破坏,生成了一种新的结构,使原有的某些精细结构在二阶导数光谱中得以表现,由此探讨其生物降解机理。

To investigate biodegradation potential of azo dye C.I.Acid Red 73 under different environmental conditions,acclimatized microbial flora having 1280±65 mg/L MLSS was used to azo dye C.I.Acid Red 73 decolorization.The optimal decolorization efficiency was under the micro-aerobic environment(static-open-incubation).Much of decolorization mainly occurred during 'log' growth period.The good decolorization activity was observed in a broad pH range(3.32～9.18),a broad temperature range(20℃～37℃) and the decolorization efficiency was above 80%.Under the micro-aerobic environment,C.I.Acid Red 73 degradation courses showed reductive cleavage of azo bond led to the formation of a new structure.Some intrinsic fine structures could be represented in second derivative spectrophotometry.The biodegradation mechanism of azo dye C.I.Acid Red 73 was proposed.