摘要
目的探讨白细胞介素(IL)-12、IL-13在儿童哮喘发病中的作用及意义。方法采用双抗体夹心ELISA法测定72例急性发作期和缓解期哮喘患儿外周血单个核细胞(PBMC)培养上清液中IL-12、IL-13水平,并同时测肺功能1秒钟用力呼气容积(FEV1)占预防值的百分比。结果直线相关分析表明,哮喘急性发作期IL-12与FEV1占预计值的百分比呈正相关(r=0.485,P<0.05),与IL-13呈负相关(r=-0.549,P<0.05);IL-13与FEV1占预计值的百分比呈负相关(r=-0.493,P<0.05)。结论提示IL-12、IL-13在哮喘的急性发病中起着重要作用,其可能是构成气道慢性炎症的各类因素之一。测定IL-12、IL-13水平为哮喘的诊断及判断病情提供较好的实验室参数。
OBJECTIVE To evaluate the role of IL-12 and IL-13 in the pathogenesis of asthmatic children and its clinical significance.METHODS The levels of IL-12 and IL-13 in the supernatant of cultured peripheral blood mononuclear cells(PBMC)were separately assessed with double antibody sandwich ELISA technoque in 72 acute and relieved asthmatic chilren.Lung ventilatory function forced expiratory volume in one second(FEV1)were measured in all patients.RESULTS Correlatin analysis showed that the level of IL-12 was positively correlated to FEV1 and negatively correlated to level of IL-13 in acute asthma attack(r1=0.458,r2=-0.549,respectively P<0.05);the level of IL-13 was negatively correlated to FEV1(r=-0.493,P<0.05).CONCLUSION The result indicates that IL-12 and IL-13 play an important role in the asthmatic attack,they may be one of the factor causing bronchial chronic inflammation.The level of IL-12 and IL-13 can be used as diagnosis and judgment of asthma.
出处
《中国初级卫生保健》
2004年第6期45-46,共2页
Chinese Primary Health Care
基金
深圳市科技局卫生医疗科技计划项目(项目编号:200304225)
