期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

诱导型一氧化氮合酶基因在3T3细胞中的转染和鉴定 被引量：1

Expression and identification of human inducible nitric oxide synthase gene in 3T3 cells
下载PDF
导出
摘要 目的 :观察人诱导型一氧化氮合酶 (iNOS)基因转染 3T3成纤维细胞的可能性。　方法 :用阳性脂质体将含人iNOS基因的真核表达载体pcDNA3.0 iNOS转染至 3T3成纤维细胞 ,用G4 18筛选 ,通过RT PCR、免疫组化的方法鉴定。　结果 :pcDNA3.0 iNOS基因转染的 3T3细胞有人iNOS基因mRNA和iNOS蛋白的表达。　结论 :iNOS基因能在 3T3成纤维细胞得到稳定转染和表达 ,能为模拟体内一氧化氮 (NO)作用提供有力的工具。 Objective:To observe the feasibility of transfection human nitric oxide synthase in 3T3 cells.Methods:The eukaryotic expression vector pcDNA3.0-iNOS was transfected into 3T3 cells.After screening with G418 and cloning,the cells were verified with RT-PCR and immunofluorescence staining. Results:the expression of iNOS mRNA and iNOS protein in 3T3 cells transfected with pcDNA3.0-iNOS was detected. Conclusion:the 3T3 cells transfected with pcDNA3.0-iNOS can be a useful tool to study the function of nitric oxide in vivo.
作者 蒲晓允 张春雷 邓均 李召权 黄辉
机构地区 第三军医大学检验系临床血液学教研室
出处 《医学研究生学报》 CAS 2004年第6期484-486,F002,共4页 Journal of Medical Postgraduates
关键词 一氧化氮 基因转染 诱导型一氧化氮合酶 Nitric oxide Gene transfer Inducible nitric oxide synthase
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献1

  • 1Bruce Demple. Signal transduction by nitric oxide in cellular stress responses[J] 2002,Molecular and Cellular Biochemistry(1):11～18

同被引文献9

  • 1Haynes V,Elfering SL,Squires R J,et al.Mitochondrial nitricoxide synthase:role in pathophysiology[J].IUBMB Life,2003,55(10-11):599-603.
  • 2Fecker LF,Eberle J,Orfanos CE,et al.Inducible nitric oxide synthase is expressed in normal human melanocytes but not in melanoma cells in response to tumor necrosis factor-alpha,interferon-gamma,and lipopolysaccharide[ J ].J Invest Dermatol,2002,118(6):1019-1025.
  • 3Lechner M,Lirk P,Rieder J.Inducible nitric oxide synthase (iNOS) in tumor biology:the two sides of the same coin[J].Semin Cancer Biol,2005,15 (4):277-289.
  • 4Wang Z,Cook T,Alber S,et al.Adenoviral gene transfer of the human inducible nitric oxide synthase gene enhances the radiation response of human colorectal cancer associated with alterations in tumor vascularity[ J ].Cancer Res,2004,64 (4):1386-1395.
  • 5Adams DJ,Levesque MC,Weinberg JB,et al.Nitric oxide enhancement of fludarabine cytotoxicity for B-CLL lymphocytes[J].Leukemia,2001,15 (12):1852-1859.
  • 6Konovalova NP,Goncharova SA,Volkova LM,et al.Nitric oxide donor increases the efficiency of cytostatic therapy and retards the development of drug resistance[ J ].Nitric Oxide,2003,8(1):59-64.
  • 7Ferry-Dumazet H,Mamani-Matsuda M,Dupouy M,et al.Nitric oxide induces the apoptosis of human BCR-ABL-positive myeloid leukemia cells:evidence for the chelation of intracellular iron[J].Leukemia,2002,16(4):708-715.
  • 8Liu S,Bishop WR,Liu M.Differential effects of cell cycle regulatory protein p21 (WAF1/Cip1) on apoptosis and sensitivity to cancer chemotherapy[ J ].Drug Resist Updat,2003,6 (4):183-195.
  • 9张春雷,邓均,蒲晓允.一氧化氮与血液细胞的凋亡和分化[J].国外医学（输血及血液学分册）,2003,26(2):164-166. 被引量：4

引证文献1

二级引证文献2

医学研究生学报
2004年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部