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慢传输型便秘模型的建立及其机制探讨 被引量:49

Establishment of an animal model of slow tramit constipation and the investigation of its mechanism
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摘要 目的 :建立实验型慢传输型便秘模型。 方法 :实验组小鼠皮下注射吗啡建立慢传输型便秘模型 ,记录小鼠粪便重量 ,利用炭沫推进试验比较实验组与对照组小鼠结肠传输功能 ;利用免疫组化技术比较两组小鼠结肠组织中Cajal细胞数量。 结果 :实验组小鼠粪便重量减轻 (P <0 .0 1) ,结肠推进率较对照组明显延长 (P <0 .0 1) ,Ca jal细胞数量较对照组明显减少 (P <0 .0 1)。 结论 :吗啡皮下注射诱导小鼠结肠慢传输型便秘模型符合疾病的基本特点 ,其发病机制可能与内源性阿片肽增多和 (或 ) Objective:To establish an animal model of slow transit constipation and the pathobiological changes in interstitial cell of Cajal in colon. Methods:The mouse model was established by subcutaneous administration of morphine. Fecal weight was recorded daily. Transit functions of intestinal movement were examined by activated charcoal suspension pushing test and the changes of interstitial cell of Cajal were observed by immunohistochemical methods. Results:Compared with the controlled mice, there was a significant decrease in fecal weight daily(P<0.01), intestinal transit ratio and number of interstitial cell of Cajal in colon tissue were significant decreased(P<0.01)in mice with morphine. Conclusions:The animal model of slow intestinal transit movement induced by morphine conforms with clinical characteristic of slow transit constipation,endogenous opium peptide may leads to reduce of interstitial cell of Cajal, and at last maybe result in slowing motility of intestine.
出处 《医学研究生学报》 CAS 2004年第6期502-504,M018,共4页 Journal of Medical Postgraduates
关键词 慢传输型便秘 动物模型 CAJAL间质细胞 吗啡 Slow transit constipation Animal model Interstitial cell of Cajal Morphine
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