异种骨衍生材料移植早期兔外周血T淋巴细胞亚群的变化

The Study of the Change of Peripheral Blood T Lymphocyte Subsets CD_4/CD_8 Ratio of Rabbit in Early Period after Transplantation of Three Kinds of Xenogeneic Bone Derived Materials

目的 :观察三种异种骨衍生材料移植早期兔外周血T淋巴细胞亚群的变化 .方法 :用复合型完全脱蛋白骨 (CFDB)、部分脱蛋白骨 (PDPB)、部分脱钙骨 (PDCB)三种异种骨衍生材料修复兔桡骨节段性缺损 ,术后 1 ,2 ,4周取材 ,用流式细胞仪检测三种材料移植早期兔外周血T淋巴细胞亚群的变化 ,并通过常规组织学检测观察 4周时三种材料的成骨作用 ,以新鲜异种骨 (FXB)和空白缺损为对照 .结果 :三种材料移植后外周血T淋巴细胞亚群CD4/CD8值在各个阶段与空白对照组无显著差异 (P >0 0 5 ) ,与FXB组有显著性差异 (P <0 0 5 ) .4周时组织形态观察可见新骨贴附三种材料向缺损方向生长 .结论 :三种材料移植早期未引起严重的免疫排斥反应 ,且不影响新骨形成 ,可望用于修复骨缺损 .

Objective:To analyze the change of peripheral blood T lymphocyte subsets CD4/CD8 ratio of rabbit in early period after transplantation of three kinds of xenogeneic bone derived materials.Methdos:Complicated fully deproteinised bone(CFDB),partially deproteinised bone(PDPB),partially decalcified bone(PDCB)were used to repair the segmental defect of the rabbits radii,Flow cytometry was used to study the change of Ilylymphocyte subsets of peripheral blood of the rabbit that in early period after 1 week,2 weeks and 4 weeks,and the osteogenesis were observed with histology after 4 weeks three xenogeneic bone derived materials being implanted,fresh xenogeneic bone(FXB)and empty defect were used as control.Results:The peripheral blood T lymphocyte subsets CD4/CD8 ratio of xenogeneic bone derived material groups had no significant difference with the empty contrast group(P>0.05),but has significant difference with FXB group(P<0.05)in all the stage.New bone formation adhesive to the material and grew toward defect after 4 weeks through histologic observation.Conclusions:Three kinds of xenogeneic bone derived materials didnt cause serious immunologic rejection in early period after transplantation;new bone formation was not affected,they could be used to repair bone defect.