急性髓细胞性白血病患者血清IL-12、IFN-Y及LDH的测定

The Clinical Significance of The Detection or Serum IL-12、IFN-Y And LDH in Patients With Acute Myelocyte Leukmia

目的 通过测定急性髓细胞性白血病(AML)患者血清IL-12、IFN-Y及LDH的变化,来探讨三者在AML的诊断、病情监测、疗效观察及预后判断中的意义.方法 采用酶联免疫(ELISA)法测定43例AML患者不同阶段血清白细胞介素-12(IL-12)、Y干扰素(IFN-Y)水平,全自动生化分析仪酶法测定乳酸脱氢酶(LDH)水平,并与正常对照比较.结果 初诊时IL-12、IFN-Y低于对照组(P<0.01,<0.05),LDH高于对照组(P<0.01);IL-12与IFN-Y呈显著正相关(r=0.498,P<0.01).完全缓解时IL-12和IFN-Y升高,LDH明显下降但仍高于对照组(P<0.01).复发时IL-12、IFN-Y再次减低,LDH再次升高,与CR组比较均有显著性差异(t,t=4.03～17.47,P<0.05),而与初诊组比较三者均无显著性差异;对化疗不敏感者,其血清IL-12、IFN-Y下降幅度及LDH升高的幅度明显高于化疗敏感者.各亚型之间IL-12、IFN-Y、LDH含量均无显著性差异(F=0.11440.530,P>0.05).结论 ①病人血清IL-12、IFN-Y、LDH与AML的发生、发展及预后密切相关.②血清IL-12、IFN-Y和LDH的监测时AML的早期复发及预后判断有一定帮助.③AML各亚型之间血清IL-12、IFN-Y和LDH的含量没有明显变化.

Objective To evaluate the level of serum IL-12,IFN-Y and LDH in patients with acute myelocyte leukemia.Methods Serum levels of IL-12 and IFN-Y were detected by ELISA method,concentration of LDH was assayed by Auto Biochemistry Analyzer before and after treatments in cases of acute myelocyte leukemia.Results The contents of IL-12 and IFN-Y were significantly lower(t=6.34,2.609,both all P<0.01),while the content of LDH was significantly higher(t=2.362,P<0.01) in acute myelocyte leukemia patients during diagnosis in comparison with that in the control group,There was a significant relationship between these two levels of IL-12 and IFN-Y(t-0.498,P<0.01).There was no conelation between the levels of LDH and IL-12,IFN-Y.In the pauents of complete remission the level of serum IL-12 and IFN-Y were significantly increased and there are no statlsuc difference compared with normal contrast(t=0.909,0.013;P>0.05);in the same time the leveI of LDH was significantly decreased.Conclusion The level of serum IL-12,IFN-Y and LDH may be related to the growth of the leukemia cells and the depress of human immune status of antitumor function.