摘要
Objective To investigate the apoptosis effect of Total alkaloids on human gastric cancer cells SGC-7901 from Capparis spinosa(C.S)and possible mechanism of it.Methods SGC-7901 cells were treated with different concentrations of the Total alkaloids in CS.MTT assay and SRB assay were used to observe the inhibitory rate of the Total alkaloids,and fluorescence microscope,flow cytometry and used to observe the influence of the Total alkaloids on cell apoptosis and cell cycle changes of SGC-7901.Results The results showed total alkaloids can inhibit the growth of human gastric adenoma cells SGC-7901.Measurements using mononuclear cell direct cytotoxicity assay(the MTT method)shows that its cytotoxic effect on SGC-7901 is strong,with IC50 being 142.895 μg·mL-1,respectively.Results from SRB assay show that the anticancer effect of Total alkaloids is cytostatic at low concentration,with LC50 for this cells being 41.271 μg·mL-1,respectively,but it becomes mainly cytotoxic at high concentration,with GI50 for SGC-7901 being 244·932 μg·mL-1,respectively.Total alkaloids can induce apoptosis in tumor cells.Forty-eight hours after they are treated with total alkaloids of different concentrations,SGC-7901 cells are stained with Hoechst33258 fluorochromes.Observation using a fluorescence microscope reveals that total alkaloids can cause the chromatin in tumor cell nuclei to condense and fragment.The nuclei condense into a uniform,dense mass and then break up.Sprouts keep on forming on the cell membrane and then dropping off,so that the cell breaks up into several apoptotic bodies of different sizes.As total alkaloids concentration is increased,these morphological changes under the microscope become more and more clear,indicating that the proportion of cells undergoing apoptosis is gradually increasing.After treating with 75,150 and 300 μg·mL-1 of the total alkaloids in C.S for 72 h,the apoptotic rates of SGC-7901 cells were 8.7%,14.309%,0.819%.Conclusions Inducing apoptosis is one of the anti-cancer mechanism of total alkaloids of C.S.
Objective To investigate the apoptosis effect of Total alkaloids on human gastric cancer cells SGC-7901 from Capparis spinosa(C.S)and possible mechanism of it.Methods SGC-7901 cells were treated with different concentrations of the Total alkaloids in CS.MTT assay and SRB assay were used to observe the inhibitory rate of the Total alkaloids,and fluorescence microscope,flow cytometry and used to observe the influence of the Total alkaloids on cell apoptosis and cell cycle changes of SGC-7901.Results The results showed total alkaloids can inhibit the growth of human gastric adenoma cells SGC-7901.Measurements using mononuclear cell direct cytotoxicity assay(the MTT method)shows that its cytotoxic effect on SGC-7901 is strong,with IC50 being 142.895 μg·mL-1,respectively.Results from SRB assay show that the anticancer effect of Total alkaloids is cytostatic at low concentration,with LC50 for this cells being 41.271 μg·mL-1,respectively,but it becomes mainly cytotoxic at high concentration,with GI50 for SGC-7901 being 244·932 μg·mL-1,respectively.Total alkaloids can induce apoptosis in tumor cells.Forty-eight hours after they are treated with total alkaloids of different concentrations,SGC-7901 cells are stained with Hoechst33258 fluorochromes.Observation using a fluorescence microscope reveals that total alkaloids can cause the chromatin in tumor cell nuclei to condense and fragment.The nuclei condense into a uniform,dense mass and then break up.Sprouts keep on forming on the cell membrane and then dropping off,so that the cell breaks up into several apoptotic bodies of different sizes.As total alkaloids concentration is increased,these morphological changes under the microscope become more and more clear,indicating that the proportion of cells undergoing apoptosis is gradually increasing.After treating with 75,150 and 300 μg·mL-1 of the total alkaloids in C.S for 72 h,the apoptotic rates of SGC-7901 cells were 8.7%,14.309%,0.819%.Conclusions Inducing apoptosis is one of the anti-cancer mechanism of total alkaloids of C.S.
作者
YU Lei1,2,JI Yu-bin1,2,MO Ke1,WANG Wei1,CUI Rong-tian1,ZOU Xiang1,2,3(1.Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Commerce,Harbin 150076,China
2.Institute of Materia Medica and Postdoctoral Programme of Harbin University of Commerce,Harbin 150076,China
3.Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2008年第S1期74-75,共2页
Journal of Shenyang Pharmaceutical University
