Ginsenoside metabolite IH 901 reduced foam cell formation in vitro

Objective To explore the roles of IH 901 on the formation of foam cell sourced from rat peritoneal macrophage.Methods The cultured rat peritoneal macrophages were incubated with ox-LDL to get foam cell as model group.Different doses of IH 901 were added into the culture medium(10 μM,25 μM,50 μM).Foam cell was stained by oil red O.The content of total cholesterol and cholesterol ester were examined to show the formation of foam cells.And the concentrations of TNF-α and IL-1 in culture supernatant were detected.The expression of NF-κB,perilipin and CD36 were measured by western blotting.Results Compared with model group,the formation of foam cell was significantly reduced by IH 901 treatment(50 μM,25 μM),while the amount of living cells of 50 μM group was lessened compared with other groups.Compared with model group,the contents of total cholesterol,cholesterol ester and CE/TC ratio were significantly reduced in IH 901 50 μM and 25 μM groups(P<0.05).Meanwhile,there was obvious difference among IH 901 groups.The concentrations of TNF-α and IL-1 in IH 901 high and middle dose groups were reduced remarkable compared with model group.The changes of expression of NF-κB,perilipin and CD36 showed the similar tendency of the results above.There were no noticeable difference between model group and IH 901 low dose group.Conclusions IH 901 could reduce the formation of foam cell sourced from rat peritoneal macrophage dose dependently.The mechanism may depend on the effects of IH 901 reducing the inflammation reaction of macrophage and the expression of perilipin.