CXCL16趋化因子在小鼠免疫性肝损伤中的作用

Involvement of CXCL16 chemokine in immunological liver injury in mice

目的 :研究CXCL1 6在免疫性肝脏损伤中的表达及其功能。方法 :运用实时定量PCR检测CXCL1 6在小鼠肝损伤模型中的表达变化 ;通过特异中和抗体的体内阻断CXCL1 6功能实验 ,观察ALT水平、肝脏病理变化、TNF α和FasL凋亡基因表达、肝脏内浸润淋巴细胞及其主要T细胞亚群的数量变化以及小鼠存活率等指标 ,研究CXCL1 6在肝脏炎症和损伤中的作用 ,并初步探讨它的可能机理。结论 :肝脏组织中CXCL1 6的上调表达介导了特异性淋巴细胞向肝脏局部组织的趋化和募集 ,并协同调节其它相关分子的表达 。

Objective:CXCL16 chemokine,with both secretory and transmembrane forms,is a recently indentified to selectively chemoattract CXCR6 + T lymphocytes.To investigate the expression pattern of CXCL16 chemokine and pathophysiological roles of anti CXCL16 antibody in injured liver induced by BCG and LPS.Methods:Expression of CXCL16 was detected in immunological liver injury induced by BCG and LPS,and neutralizing anti CXCL16 monoclonal antibody(mAb)was further administrated in vivo to investigate its roles in hepatic necrosis,expression of apoptosis molecules,infiltration of hepatic lymphocytes and survive rate of mice.The dominant hepatic T lymphocytes populations were also analyzed to study the mechanism of CXCL16 in liver injury.Conclusion:These findings suggest that CXCL16 might play an important role in massive hepatic necrosis in BCG LPS induced liver injury by recruitment of intrahepatic specific lymphocytes and regulation of injury related molecules expression.